轉(zhuǎn)錄調(diào)控-DAP-seq

100+物種，2000+轉(zhuǎn)錄因子的DAP-seq實(shí)戰(zhàn)經(jīng)驗(yàn)，無(wú)需抗體，高通量鑒定轉(zhuǎn)錄因子的下游基因，藍(lán)景科信已助力客戶在許多期刊發(fā)表文章，例如：Molecular Plant，The Plant Cell，Plant Physiology，Plant Biotechnology Journal，Journal of Integrative Plant Biology，New Phytologist等。

在功能基因組學(xué)和表觀遺傳學(xué)研究中，轉(zhuǎn)錄因子結(jié)合位點(diǎn)（TFBS）的發(fā)掘一直是研究熱點(diǎn)。傳統(tǒng)的ChIP-seq（染色質(zhì)免疫共沉淀測(cè)序）方法，在抗體質(zhì)量很好的情況下能夠有效檢測(cè)到TFBS。然而，好的抗體可遇不可求，這限制了ChIP-seq更廣泛的應(yīng)用。

DAP-seq技術(shù)的出現(xiàn)，使TFBS 的研究不再局限于物種，不再受抗體質(zhì)量的限制，為生命科學(xué)領(lǐng)域轉(zhuǎn)錄因子的研究提供了新的有效工具。

合作案例：

Yao J, Shen Z, Zhang Y, Wu X, Wang J, Sa G, Zhang Y, Zhang H, Deng C, Liu J, Hou S, Zhang Y, Zhang Y, Zhao N, Deng S, Lin S, Zhao R, Chen S. Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance. J Exp Bot. 2020 Feb 19;71(4):1527-1539. doi: 10.1093/jxb/erz493. PMID: 31680166; PMCID: PMC7031066.

Tang N, Cao Z, Yang C, Ran D, Wu P, Gao H, He N, Liu G, Chen Z. A R2R3-MYB transcriptional activator LmMYB15 regulates chlorogenic acid biosynthesis and phenylpropanoid metabolism in Lonicera macranthoides. Plant Sci. 2021 Jul;308:110924. doi: 10.1016/j.plantsci.2021.110924. Epub 2021 Apr 29. PMID: 34034872.

Yu Z, Chen X, Li Y, Shah SHA, Xiao D, Wang J, Hou X, Liu T, Li Y. ETHYLENE RESPONSE FACTOR 070 inhibits flowering in Pak-choi by indirectly impairing BcLEAFY expression. Plant Physiol. 2024 Jan 25:kiae021. doi: 10.1093/plphys/kiae021. Epub ahead of print. PMID: 38269601.

Cao X, Li X, Su Y, Zhang C, Wei C, Chen K, Grierson D, Zhang B. Transcription factor PpNAC1 and DNA demethylase PpDML1 synergistically regulate peach fruit ripening. Plant Physiol. 2023 Nov 22:kiad627. doi: 10.1093/plphys/kiad627. Epub ahead of print. PMID: 37992120.

Zhao H, Wan S, Huang Y, Li X, Jiao T, Zhang Z, Ma B, Zhu L, Ma F, Li M. The transcription factor MdBPC2 alters apple growth and promotes dwarfing by regulating auxin biosynthesis. Plant Cell. 2024 Feb 26;36(3):585-604. doi: 10.1093/plcell/koad297. PMID: 38019898; PMCID: PMC10896295.

Meiran Li1, Mingwei Chen, Yongli Zhang, Longgang Zhao, Jiancheng Zhang, Hui Song, Identification of the target genes of AhTWRKY24 and AhTWRKY106 transcription factors reveals their regulatory network in Arachis hypogaea cv. Tifrunner using DAP-seq[J].Oil Crop Science.2023,Vol.8(No.2)：89-96.

Liu P, Bu C, Chen P, El-Kassaby YA, Zhang D, Song Y. Enhanced genome-wide association reveals the role of YABBY11-NGATHA-LIKE1 in leaf serration development of Populus. Plant Physiol. 2023 Mar 17;191(3):1702-1718. doi: 10.1093/plphys/kiac585. PMID: 36535002; PMCID: PMC10022644.

Yuan J, Liu X, Zhao H, Wang Y, Wei X, Wang P, Zhan J, Liu L, Li F, Ge X. GhRCD1 regulates cotton somatic embryogenesis by modulating the GhMYC3-GhMYB44-GhLBD18 transcriptional cascade. New Phytol. 2023 Oct;240(1):207-223. doi: 10.1111/nph.19120. Epub 2023 Jul 11. PMID: 37434324.

Dong, Q., Tian, Y., Zhang, X., Duan, D., Zhang, H., Yang, K., Jia, P., Luan, H., Guo, S., Qi, G., Mao, K., & Ma, F. (2023). Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple. Horticultural Plant Journal.

Fang Y, Wang D, Xiao L, Quan M, Qi W, Song F, Zhou J, Liu X, Qin S, Du Q, Liu Q, El-Kassaby YA, Zhang D. Allelic variation in transcription factor PtoWRKY68 contributes to drought tolerance in Populus. Plant Physiol. 2023 Aug 31;193(1):736-755. doi: 10.1093/plphys/kiad315. PMID: 37247391; PMCID: PMC10469405.

Zhu J, Wei X, Yin C, Zhou H, Yan J, He W, Yan J, Li H. ZmEREB57 regulates OPDA synthesis and enhances salt stress tolerance through two distinct signalling pathways in Zea mays. Plant Cell Environ. 2023 Sep;46(9):2867-2883. doi: 10.1111/pce.14644. Epub 2023 Jun 16. PMID: 37326336.

Han P, Hua Z, Zhao Y, Huang L, Yuan Y. PuCRZ1, an C2H2 transcription factor from Polyporus umbellatus, positively regulates mycelium response to osmotic stress. Front Microbiol. 2023 Apr 6;14:1131605. doi: 10.3389/fmicb.2023.1131605. PMID: 37089566; PMCID: PMC10115967.

Zhang S, Wang L, Yao J, Wu N, Ahmad B, van Nocker S, Wu J, Abudureheman R, Li Z, Wang X. Control of ovule development in Vitis vinifera by VvMADS28 and interacting genes. Hortic Res. 2023 Apr 13;10(6):uhad070. doi: 10.1093/hr/uhad070. PMID: 37293531; PMCID: PMC10244803.

Wang L, Tian T, Liang J, Li R, Xin X, Qi Y, Zhou Y, Fan Q, Ning G, Becana M, Duanmu D. A transcription factor of the NAC family regulates nitrate-induced legume nodule senescence. New Phytol. 2023 Jun;238(5):2113-2129. doi: 10.1111/nph.18896. Epub 2023 Apr 9. PMID: 36945893.

Li M, Hou L, Zhang C, Yang W, Liu X, Zhao H, Pang X, Li Y. Genome-Wide Identification of Direct Targets of ZjVND7 Reveals the Putative Roles of Whole-Genome Duplication in Sour Jujube in Regulating Xylem Vessel Differentiation and Drought Tolerance. Front Plant Sci. 2022 Feb 4;13:829765. doi: 10.3389/fpls.2022.829765. PMID: 35185994; PMCID: PMC8854171.

Yang Z, Liu Z, Ge X, Lu L, Qin W, Qanmber G, Liu L, Wang Z, Li F. Brassinosteroids regulate cotton fiber elongation by modulating very-long-chain fatty acid biosynthesis. Plant Cell. 2023 May 29;35(6):2114-2131. doi: 10.1093/plcell/koad060. PMID: 36861340; PMCID: PMC10226586.

Sun Y, Han Y, Sheng K, Yang P, Cao Y, Li H, Zhu QH, Chen J, Zhu S, Zhao T. Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bickii. Mol Plant. 2023 Apr 3;16(4):694-708. doi: 10.1016/j.molp.2023.02.005. Epub 2023 Feb 10. PMID: 36772793.

Liu Y, Liu Q, Li X, Zhang Z, Ai S, Liu C, Ma F, Li C. MdERF114 enhances the resistance of apple roots to Fusarium solani by regulating the transcription of MdPRX63. Plant Physiol. 2023 Jul 3;192(3):2015-2029. doi: 10.1093/plphys/kiad057. PMID: 36721923; PMCID: PMC10315273.

Liu YN, Wu FY, Tian RY, Shi YX, Xu ZQ, Liu JY, Huang J, Xue FF, Liu BY, Liu GQ. The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi. Commun Biol. 2023 Jan 3;6(1):1. doi: 10.1038/s42003-022-04154-6. PMID: 36596887; PMCID: PMC9810662.

Liu L, Chen G, Li S, Gu Y, Lu L, Qanmber G, Mendu V, Liu Z, Li F, Yang Z. A brassinosteroid transcriptional regulatory network participates in regulating fiber elongation in cotton. Plant Physiol. 2023 Mar 17;191(3):1985-2000. doi: 10.1093/plphys/kiac590. PMID: 36542688; PMCID: PMC10022633.

Bi Y, Wang H, Yuan X, Yan Y, Li D, Song F. The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6. J Integr Plant Biol. 2023 Mar;65(3):854-875. doi: 10.1111/jipb.13399. Epub 2023 Feb 23. PMID: 36308720.

Guo X, Yu X, Xu Z, Zhao P, Zou L, Li W, Geng M, Zhang P, Peng M, Ruan M. CC-type glutaredoxin, MeGRXC3, associates with catalases and negatively regulates drought tolerance in cassava (Manihot esculenta Crantz). Plant Biotechnol J. 2022 Dec;20(12):2389-2405. doi: 10.1111/pbi.13920. Epub 2022 Sep 14. PMID: 36053917; PMCID: PMC9674314.

Tang N, Cao Z, Yang C, Ran D, Wu P, Gao H, He N, Liu G, Chen Z. A R2R3-MYB transcriptional activator LmMYB15 regulates chlorogenic acid biosynthesis and phenylpropanoid metabolism in Lonicera macranthoides. Plant Sci. 2021 Jul;308:110924. doi: 10.1016/j.plantsci.2021.110924. Epub 2021 Apr 29. PMID: 34034872.

Chai Z, Fang J, Huang C, Huang R, Tan X, Chen B, Yao W, Zhang M. A novel transcription factor, ScAIL1, modulates plant defense responses by targeting DELLA and regulating gibberellin and jasmonic acid signaling in sugarcane. J Exp Bot. 2022 Nov 2;73(19):6727-6743. doi: 10.1093/jxb/erac339. PMID: 35986920.

Li R, Zheng W, Yang R, Hu Q, Ma L, Zhang H. OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice. Plant J. 2022 Oct;112(1):151-171. doi: 10.1111/tpj.15937. Epub 2022 Aug 19. PMID: 35942609.

Li Q, Zhou L, Chen Y, Xiao N, Zhang D, Zhang M, Wang W, Zhang C, Zhang A, Li H, Chen J, Gao Y. Phytochrome interacting factor regulates stomatal aperture by coordinating red light and abscisic acid. Plant Cell. 2022 Oct 27;34(11):4293-4312. doi: 10.1093/plcell/koac244. PMID: 35929789; PMCID: PMC9614506.

Luo M, Lu B, Shi Y, Zhao Y, Wei Z, Zhang C, Wang Y, Liu H, Shi Y, Yang J, Song W, Lu X, Fan Y, Xu L, Wang R, Zhao J. A newly characterized allele of ZmR1 increases anthocyanin content in whole maize plant and the regulation mechanism of different ZmR1 alleles. Theor Appl Genet. 2022 Sep;135(9):3039-3055. doi: 10.1007/s00122-022-04166-0. Epub 2022 Jul 5. PMID: 35788748.

Wei H, Xu H, Su C, Wang X, Wang L. Rice CIRCADIAN CLOCK ASSOCIATED 1 transcriptionally regulates ABA signaling to confer multiple abiotic stress tolerance. Plant Physiol. 2022 Sep 28;190(2):1057-1073. doi: 10.1093/plphys/kiac196. PMID: 35512208; PMCID: PMC9516778.

相關(guān)服務(wù)：

1、 凝膠阻滯實(shí)驗(yàn)（EMSA）：DAP-seq后續(xù)驗(yàn)證服務(wù)。

2、 酵母單雜交：DAP-seq后續(xù)驗(yàn)證服務(wù)

3、 ChIP-seq：高效檢測(cè)重組蛋白、轉(zhuǎn)錄因子在基因組的結(jié)合位點(diǎn)

4、 DAP-seq與RNA-seq聯(lián)合分析： 分析轉(zhuǎn)錄因子的靶基因在RNA-seq數(shù)據(jù)中的表達(dá)變化，深入挖掘DAP-seq和RNA-seq測(cè)序數(shù)據(jù)，增加轉(zhuǎn)錄組測(cè)序的分析深度。

5、 DNA-pull down：鑒定與DNA結(jié)合的蛋白。

6、 精美的論文圖片設(shè)計(jì)與制作：專業(yè)設(shè)計(jì)師，設(shè)計(jì)精美論文插圖，提升論文的嚴(yán)謹(jǐn)性和美觀度。

轉(zhuǎn)錄調(diào)控-DAP-seq

DAP-seq是基于DNA親和純化，通過(guò)體外表達(dá)轉(zhuǎn)錄因子鑒定TFBS的技術(shù)，具有不受抗體和物種限制，且高通量的優(yōu)勢(shì)，自該技術(shù)問(wèn)世以來(lái)，已被廣泛應(yīng)用于轉(zhuǎn)錄調(diào)控和表觀組學(xué)的研究。能幫助您快速找到轉(zhuǎn)錄因子的結(jié)合位點(diǎn)，尋找轉(zhuǎn)錄因子調(diào)控的靶基因。

藍(lán)景科信河北生物科技有限公司是一家專業(yè)從事生命科學(xué)產(chǎn)品和技術(shù)開(kāi)發(fā)的公司。本公司擁有雄厚的技術(shù)實(shí)力，創(chuàng)始團(tuán)隊(duì)均具有博士學(xué)位，來(lái)自于國(guó)內(nèi)的清華大學(xué)、中國(guó)農(nóng)業(yè)大學(xué)和美國(guó)麻省理工學(xué)院。公司立足保定，放眼中外，以嚴(yán)謹(jǐn)?shù)目茖W(xué)態(tài)度，不斷滿足生命科學(xué)領(lǐng)域的市場(chǎng)需求。